pdaB [2017-8-11 16:0:3]

polysaccharide deacetylase

locus: BSU01570

pI: 9.18

mw: 28.17 kDa

protein length: 254 aa

gene length: 762 bp

function: spore cortex formation

product: polysaccharide deacetylase

essential: no

synonyms: ybaN

Genomic Context

categories

• [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.4|Sporulation proteins/ other]

Gene

Coordinates: 159,779 → 160,543

Phenotypes of a mutant

• ''[gene|BBF22E368CB091678083934FD42BB11EA30B29DD|pdaB] [gene|BB0443D95581DCA66EDA390842945F105A71A371|ytrI]'' and ''[gene|BBF22E368CB091678083934FD42BB11EA30B29DD|pdaB] [gene|D5B9ACB1E447A893C99A4ACE1DA95EB38BCF971B|ytrH]'' mutants are deficient in sporulation
• inactivation of ''[gene|BBF22E368CB091678083934FD42BB11EA30B29DD|pdaB]'' reduces sporulation efficiency to 5% that of wild type cells [Pubmed|26735940]

The protein

Protein family

• polysaccharide deacetylase family (according to Swiss-Prot)

[SW|Localization]

• forespore (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|BBF22E368CB091678083934FD42BB11EA30B29DD|pdaB]
  description: [Pubmed|12662922]

  sigma factors

  • [protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE]: sigma factor, [Pubmed|15699190,12662922], in [regulon|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE regulon]

  regulatory mechanism

  • [protein|90DCE4F286D2C151D8BDBF0E024E6BBEC94E2397|SpoIIID]: repression, [Pubmed|12662922], in [regulon|90DCE4F286D2C151D8BDBF0E024E6BBEC94E2397|SpoIIID regulon]

  regulation

  • expressed during sporulation in the mother cell [Pubmed|12662922]

  additional information

  • the mRNA is very stable (half-life > 15 min) [http://www.ncbi.nlm.nih.gov/sites/entrez/12884008 PubMed]

Biological materials

Mutant

• MGNA-B947 (ybaN::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1946 NBRP B. subtilis, Japan]

References

• 12662922,12884008,15547282,15598884,26735940